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- Aerobic, fastidious organism.
- Difficult to cultivate.
- Grows very slowly (≥2 days to detect any colonies and longer to identify species). Colonies have "fried egg" appearance on agar (more dense in the center and less dense in the periphery).
- Differ from bacteria due to smaller size, presence of sterols in membrane and lack of a cell wall. The latter makes them intrinsically resistant to penicillin and other agents that target the cell wall.
- Colonizes male and female genitourinary tract.