Johns Hopkins ABX Guide
Klebsiella granulomatis (Granuloma inguinale, Donovanosis)
MICROBIOLOGY
- Klebsiella granulomatis (formerly known as Calymmatobacterium granulomatis) is a member of the family Enterobacteriaceae.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- Pleomorphic, intracellular (macrophages > neutrophils), Gram-negative bacillus surrounded by a well-defined bipolar staining capsule (seen with Wright, Giemsa or Leishman stain)
- The organism has a safety-pin appearance with stain under microscopy.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- The organism is difficult to demonstrate microbiologically because it does not grow on any standard microbiological laboratory media.
- Culture achieved in the research settings using peripheral blood mononuclear cells cultivated with fetal calf serum after exposure to vancomycin and metronidazole for decontamination.
- Culture also has been successful on cycloheximide-treated Hep-2 cell monolayers in RPMI 1640 medium, supplemented with fetal calf serum, penicillin, and vancomycin.
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Last updated: May 11, 2022
Citation
Hynes, Noreen A. "Klebsiella Granulomatis (Granuloma Inguinale, Donovanosis)." Johns Hopkins ABX Guide, The Johns Hopkins University, 2022. Pediatrics Central, peds.unboundmedicine.com/pedscentral/view/Johns_Hopkins_ABX_Guide/540301/all/Klebsiella_granulomatis__Granuloma_inguinale__Donovanosis_.
Hynes NA. Klebsiella granulomatis (Granuloma inguinale, Donovanosis). Johns Hopkins ABX Guide. The Johns Hopkins University; 2022. https://peds.unboundmedicine.com/pedscentral/view/Johns_Hopkins_ABX_Guide/540301/all/Klebsiella_granulomatis__Granuloma_inguinale__Donovanosis_. Accessed September 27, 2023.
Hynes, N. A. (2022). Klebsiella granulomatis (Granuloma inguinale, Donovanosis). In Johns Hopkins ABX Guide. The Johns Hopkins University. https://peds.unboundmedicine.com/pedscentral/view/Johns_Hopkins_ABX_Guide/540301/all/Klebsiella_granulomatis__Granuloma_inguinale__Donovanosis_
Hynes NA. Klebsiella Granulomatis (Granuloma Inguinale, Donovanosis) [Internet]. In: Johns Hopkins ABX Guide. The Johns Hopkins University; 2022. [cited 2023 September 27]. Available from: https://peds.unboundmedicine.com/pedscentral/view/Johns_Hopkins_ABX_Guide/540301/all/Klebsiella_granulomatis__Granuloma_inguinale__Donovanosis_.
* Article titles in AMA citation format should be in sentence-case
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