Klebsiella granulomatis (Granuloma inguinale, Donovanosis)
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MICROBIOLOGY
- Klebsiella granulomatis (formerly known as Calymmatobacterium granulomatis) is a member of the family Enterobacteriaceae.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- Pleomorphic, intracellular (macrophages > neutrophils), Gram-negative bacillus surrounded by a well-defined bipolar staining capsule (seen with Wright, Giemsa or Leishman stain)
- Organism has safety-pin appearance with stain under microscopy.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- Organism difficult to demonstrate microbiologically because it does not grow on any standard microbiological laboratory media.
- Culture achieved in the research settings using peripheral blood mononuclear cells cultivated with fetal calf serum after exposure to vancomycin and metronidazole for decontamination.
- Culture also has been successful on cyclohexamide-treated Hep-2 cell monolayers in RPMI 1640 medium, supplemented with fetal calf serum, penicillin, and vancomycin.
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MICROBIOLOGY
- Klebsiella granulomatis (formerly known as Calymmatobacterium granulomatis) is a member of the family Enterobacteriaceae.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- Pleomorphic, intracellular (macrophages > neutrophils), Gram-negative bacillus surrounded by a well-defined bipolar staining capsule (seen with Wright, Giemsa or Leishman stain)
- Organism has safety-pin appearance with stain under microscopy.
- Reclassification is based upon nucleotide relatedness to other Klebsiella spp. especially to K. rhinoscleromatis, another tropical infection (nasal).
- Organism difficult to demonstrate microbiologically because it does not grow on any standard microbiological laboratory media.
- Culture achieved in the research settings using peripheral blood mononuclear cells cultivated with fetal calf serum after exposure to vancomycin and metronidazole for decontamination.
- Culture also has been successful on cyclohexamide-treated Hep-2 cell monolayers in RPMI 1640 medium, supplemented with fetal calf serum, penicillin, and vancomycin.
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