Klebsiella species
Lisa A. Spacek, M.D., Ph.D.
MICROBIOLOGY
MICROBIOLOGY
MICROBIOLOGY
- Gram-negative, lactose-fermenting aerobic bacilli [Fig 1] of Enterobacterales order, Enterobacteriaceae family.
- K. pneumoniae, K. oxytoca and K. granulomatis.
- K. ozaenae and K. rhinoscleromatis are non-fermenting subspecies.[10]
- Forms highly mucoid colonies with polysaccharide capsule [Fig 2], a virulence factor that inhibits phagocytosis.
- Colonizes mammalian intestinal and respiratory tracts.[9]
- Easily cultured on non-selective media for sterile specimens or MacConkey agar for contaminated specimens.
- Contaminates sinks[7] and causes nosocomial outbreaks, esp. K. pneumoniae, K. oxytoca.[11]
- Resistance issues[14]
- Beta-lactamases are constitutive, usually produced at low levels, and confer resistance against ampicillin, amoxicillin and ticarcillin.
- Few klebsiellae lack these beta-lactamases.
- Extended-spectrum beta-lactamases (ESBLs) may be plasmid-mediated or chromosomal, confer multidrug resistance (TEM or SHV types), and are detected by in vitro resistance to ceftazidime and aztreonam.
- CTX-M type ESBLs are the most prevalent ESBL types worldwide and are commonly associated with ST131 E. coli.[16]
- Non-susceptibility to ceftriaxone is often used as a proxy for ESBL production.[6]
- Carbapenemases:
- Klebsiella pneumoniae carbapenemase, KPC (Ambler Class A): confers broad resistance and is associated with mortality rate >50%.[13]
- Many isolates in the U.S. are sequence type, ST258, a biologically fit lineage that is able to cause outbreaks.[8]
- The phenotype includes strong potentiation of meropenem by vaborbactam.[8]
- Metallo-beta-lactamases (Ambler Class B): types include IMP (imipenemase), VIM (Verona integron-encoded MBL), and NDM-1 (New Delhi metallo-beta-lactamase).
- Require zinc ions in the active site for activity.
- IMP and VIM are not resistant to aztreonam.
- NDM-1 presence is strongly suggested by combined resistance to meropenem, ceftazidime, and cefepime together with clear susceptibility to aztreonam.[17]
- OXA-type carbapenemases (Ambler Class D): include OXA-48, weakly hydrolyze carbapenems, broad-spectrum cephalosporins, and aztreonam; express resistance or decreased susceptibility to carbapenems.
- Often associated with Acinetobacter baumannii.
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